The SMAD2 Polyclonal Antibody is a valuable tool for researchers studying SMAD2, a key signaling protein involved in the TGF-beta pathway. This antibody, produced using rabbit immunization, demonstrates high specificity and sensitivity for detecting SMAD2 in human samples, making it suitable for Western blot applications. By binding to the SMAD2 protein, this antibody allows for the visualization and analysis of SMAD2 expression in various cell types, facilitating research in cell signaling, developmental biology, and cancer biology.SMAD2 is a critical mediator of TGF-beta signaling, regulating processes such as cell growth, differentiation, and apoptosis.
Dysregulation of the TGF-beta pathway, including alterations in SMAD2 activity, has been implicated in various diseases, including cancer, fibrosis, and developmental disorders. Therefore, studying SMAD2 expression and function is essential for understanding the mechanisms underlying these conditions and identifying potential therapeutic targets for intervention.In summary, the SMAD2 Polyclonal Antibody offers a reliable and effective tool for investigating SMAD2 signaling in cellular processes and disease states. Its specificity and versatility make it a valuable asset for researchers seeking to elucidate the role of SMAD2 in health and disease.
Product Name:
Smad2 Polyclonal Antibody
SKU:
CAB0440
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 175-270 of human Smad2 (NP_005892.1).
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
4087
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Smad2 Rabbit pAb (CAB0440).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
