The SMAD1/SMAD5/SMAD9 Polyclonal Antibody is a valuable tool for research involving members of the SMAD protein family, which play a crucial role in cell signaling and transcriptional regulation. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to SMAD1, SMAD5, and SMAD9 proteins, this antibody enables accurate detection and analysis in various cell types, making it an essential reagent for studies in developmental biology, stem cell research, and cancer biology.SMAD proteins are key components of the TGF-β signaling pathway, essential for controlling processes such as cell growth, differentiation, and apoptosis.
Dysregulation of SMAD signaling has been linked to various diseases, including cancer, fibrosis, and developmental disorders. Understanding the role of SMAD1, SMAD5, and SMAD9 in these pathways is vital for developing targeted therapies that can modulate their activity and potentially treat these conditions effectively. With its high specificity and sensitivity, the SMAD1/SMAD5/SMAD9 Polyclonal Antibody is a valuable asset for researchers aiming to unravel the complexities of SMAD-mediated signaling in health and disease.
Product Name:
Smad1/Smad5/Smad9 Polyclonal Antibody
SKU:
CAB23208
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 19-125 of human Smad1/Smad5/Smad9(NP_005891.1).
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.
Purification Method:
Affinity purification
Gene ID:
4086/4090/4093
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C2C12 cells using Smad1/Smad5/Smad9 Rabbit pAb(CAB23208) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
