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Smad1/Smad5/Smad9 Polyclonal Antibody (CAB23208)

SKU:
CAB23208
$155
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Description

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Product Name:Smad1/Smad5/Smad9 Polyclonal Antibody
SKU:CAB23208
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 19-125 of human Smad1/Smad5/Smad9(NP_005891.1).
Sequence:GWKQ GDEE EKWA EKAV DALV KKLK KKKG AMEE LEKA LSCP GQPS NCVT IPRS LDGR LQVS HRKG LPHV IYCR VWRW PDLQ SHHE LKPL ECCE FPFG SKQK EVCI NPY
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Synonyms:Smad1/Smad5/Smad9
Positive Sample:C2C12
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus
Calculated MW:52kDa
Observed MW:52kDa

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.

Purification Method:Affinity purification
Gene ID:4086/4090/4093
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from C2C12 cells using Smad1/Smad5/Smad9 Rabbit pAb(CAB23208) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.

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