Smad1 Polyclonal Antibody (CAB21734)
- SKU:
- CAB21734
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
- IHC
- IF
Description
| Product Name: | Smad1 Polyclonal Antibody |
| SKU: | CAB21734 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-240 of human Smad1 (NP_001003688.1). |
| Sequence: | WKQG DEEE KWAE KAVD ALVK KLKK KKGA MEEL EKAL SCPG QPSN CVTI PRSL DGRL QVSH RKGL PHVI YCRV WRWP DLQS HHEL KPLE CCEF PFGS KQKE VCIN PYHY KRVE SPVL PPVL VPRH SEYN PQHS LLAQ FRNL GQNE PHMP LNAT FPDS FQQP NSHP FPHS PNSS YPNS PGSS SSTY PHSP TSSD PGSP FQMP ADTP PPAY LPPE DPMT QDGS Q |
| Tested Applications: | WB IHC-P IF/ICC ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 |
| Synonyms: | BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1; d1 |
| Positive Sample: | HeLa,C2C12,ECV-304,Mouse lung |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, Nucleus. |
| Calculated MW: | 52kDa |
| Observed MW: | 60kDa |
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
| Purification Method: | Affinity purification |
| Gene ID: | 4086 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using [KO Validated] Smad1 Rabbit pAb (CAB21734) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s. |
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