The SMAC/Diablo Rabbit Monoclonal Antibody (CAB8889) is a valuable tool for researchers studying apoptosis and cell death pathways. SMAC/Diablo is a protein that functions as an inhibitor of apoptosis and plays a crucial role in regulating cell death by antagonizing inhibitors of apoptosis proteins (IAPs). This antibody, generated in rabbits, is highly specific and reactive with human samples, making it an ideal choice for experiments in Western blotting, immunohistochemistry, and flow cytometry. It binds specifically to the SMAC/Diablo protein, allowing for precise detection and analysis in a variety of cell types and tissues.
Research on SMAC/Diablo is essential for understanding the mechanisms of cell death and survival, particularly in the context of cancer and neurodegenerative diseases. By targeting SMAC/Diablo, researchers aim to develop therapeutic strategies that can promote apoptosis in cancer cells or protect neurons from degeneration.Overall, the SMAC/Diablo Rabbit Monoclonal Antibody is a reliable tool for investigating the intricate pathways involved in apoptosis and cell death, with the potential to contribute valuable insights to the field of biomedical research.
Product Name:
Smac/Diablo Rabbit Monoclonal Antibody
SKU:
CAB8889
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 56-239 of human Smac/Diablo (NP_063940.1).
This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
56616
Clone Number:
ARC1340
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Smac/Diablo Rabbit mAb (CAB8889) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
