The SLIT2 Polyclonal Antibody (CAB15353) is a powerful tool for researchers studying SLIT2, a secreted glycoprotein that plays a crucial role in axon guidance and neuronal migration during development. This antibody, produced in rabbits, exhibits high specificity and sensitivity for SLIT2 in human samples, making it ideal for applications such as Western blotting.SLIT2 is known to interact with its receptors ROBO1 and ROBO2 to regulate neuronal growth and connectivity in the central nervous system. Aberrant SLIT2 signaling has been implicated in various neurological disorders, including autism and schizophrenia.
By targeting SLIT2 with this antibody, researchers can gain insights into the molecular mechanisms underlying these conditions and potentially identify new therapeutic targets.Overall, the SLIT2 Polyclonal Antibody is a valuable tool for investigating the role of SLIT2 in neurodevelopmental processes and neurological disorders, offering a deeper understanding of the complex mechanisms at play in the brain.
Product Name:
Slit2 Rabbit Polyclonal Antibody
SKU:
CAB15353
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1330-1529 of human Slit2 (NP_004778.1).
This gene encodes a member of the slit family of secreted glycoproteins, which are ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. Members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Proteolytic processing of this protein gives rise to an N-terminal fragment that contains the four leucine-rich repeats and five epidermal growth factor repeats and a C-terminal fragment that contains four epidermal growth factor repeats and the cysteine knot. Both full length and cleaved proteins are secreted extracellularly and can function in axon repulsion as well as other specific processes. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
9353
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Slit2 Rabbit pAb (CAB15353).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
