The SLC4A1 Polyclonal Antibody (CAB17391) is a valuable tool for researchers studying the SLC4A1 protein, an important membrane transporter involved in ion transport and pH regulation. This antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples, making it ideal for Western blot applications.SLC4A1, also known as Band 3, is a crucial component of red blood cells, playing a key role in maintaining cell shape and facilitating gas exchange. Dysregulation of SLC4A1 has been linked to various disorders including hereditary spherocytosis and distal renal tubular acidosis.
By targeting SLC4A1 with this antibody, researchers can delve into its function and role in such conditions, paving the way for potential therapeutic interventions.Furthermore, understanding the mechanisms by which SLC4A1 operates can provide insights into its potential as a therapeutic target for conditions involving abnormal pH regulation, such as cancer and metabolic disorders. The SLC4A1 Polyclonal Antibody (CAB17391) is a valuable tool for unraveling the complexities of SLC4A1 biology and its implications in health and disease.
Product Name:
SLC4A1 Rabbit Polyclonal Antibody
SKU:
CAB17391
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-353 of human SLC4A1 (NP_000333.1).
basolateral plasma membrane, blood microparticle, cortical cytoskeleton, cytoplasmic side of plasma membrane, extracellular exosome, plasma membrane, Z disc
Calculated MW:
102kDa
Observed MW:
102kDa
The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system. Southeast Asian ovalocytosis (SAO, Melanesian ovalocytosis) results from the heterozygous presence of a deletion in the encoded protein and is common in areas where Plasmodium falciparum malaria is endemic. One null mutation in this gene is known, resulting in very severe anemia and nephrocalcinosis.
Purification Method:
Affinity purification
Gene ID:
6521
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SLC4A1 Rabbit pAb (CAB17391) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
