Similarly, the SLC46A1 Polyclonal Antibody (CAB7397) is a valuable tool for research involving SLC46A1, a key protein involved in the transport of folate across cellular membranes. This antibody, also raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.SLC46A1, also known as the proton-coupled folate transporter (PCFT), is essential for the uptake of folate, a crucial vitamin for DNA synthesis and cell growth. Dysregulation of folate transport has been implicated in various diseases, including cancer and neurological disorders. The SLC46A1 Polyclonal Antibody enables the detection and analysis of SLC46A1 protein levels in different cell types, making it a valuable tool for research in the fields of biochemistry, molecular biology, and pharmacology.
By studying the function and regulation of SLC46A1, researchers can gain insights into how folate transport contributes to health and disease, paving the way for potential therapeutic strategies targeting this pathway. The SLC46A1 Polyclonal Antibody is a reliable instrument for advancing our understanding of folate transport mechanisms and their implications for human health.
Product Name:
SLC46A1 Polyclonal Antibody
SKU:
CAB7397
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human SLC46A1 (NP_001229295.1).
This gene encodes a transmembrane proton-coupled folate transporter protein that facilitates the movement of folate and antifolate substrates across cell membranes, optimally in acidic pH environments. This protein is also expressed in the brain and choroid plexus where it transports folates into the central nervous system. This protein further functions as a heme transporter in duodenal enterocytes, and potentially in other tissues like liver and kidney. Its localization to the apical membrane or cytoplasm of intestinal cells is modulated by dietary iron levels. Mutations in this gene are associated with autosomal recessive hereditary folate malabsorption disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Purification Method:
Affinity purification
Gene ID:
113235
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using SLC46A1 Rabbit pAb (CAB7397) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
