The SLC16A3 Polyclonal Antibody (CAB10548) is a valuable tool for researchers investigating SLC16A3, a member of the SLC16A family of transporters involved in the transport of monocarboxylates such as lactate and pyruvate across cell membranes. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the SLC16A3 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for studies in metabolism, cancer biology, and metabolic diseases.
SLC16A3, also known as MCT4, plays a crucial role in cancer metabolism by facilitating the export of lactate from highly glycolytic cancer cells, contributing to tumor growth and progression. Its involvement in lactate transport and pH regulation in tumor microenvironments makes it a promising target for the development of novel cancer therapies. Research on SLC16A3 can provide valuable insights into the metabolic adaptations of cancer cells and may lead to the identification of new treatment strategies targeting metabolic vulnerabilities in cancer.
Product Name:
MCT4/SLC16A3 Rabbit Polyclonal Antibody
SKU:
CAB10548
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 410-465 of human MCT4/SLC16A3 (NP_004198.1).
Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton-linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family-16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found. The MCTs, which include MCT1 (SLC16A1; MIM 600682) and MCT2 (SLC16A7; MIM 603654), are characterized by 12 predicted transmembrane domains (Price et al., 1998 [PubMed 9425115]).
Purification Method:
Affinity purification
Gene ID:
9123
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using MCT4/SLC16A3 Rabbit pAb (CAB10548) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
