The SLC12A1 Polyclonal Antibody (CAB12999) is a valuable tool for researchers studying SLC12A1, a key transporter protein involved in ion transport and regulation of cell volume. This antibody, generated in rabbits, exhibits high specificity and sensitivity for human samples, making it suitable for use in Western blot applications. By binding to the SLC12A1 protein, this antibody enables researchers to analyze and detect SLC12A1 expression in a variety of cell types.SLC12A1, also known as the sodium-potassium-chloride cotransporter 2 (NKCC2), plays a crucial role in maintaining electrolyte balance and blood pressure regulation.
Dysregulation of SLC12A1 has been linked to various kidney disorders and hypertension, making it a promising target for therapeutic interventions in these conditions. By studying the function of SLC12A1, researchers can gain insights into its role in kidney physiology and the development of potential treatment strategies for related diseases.
Product Name:
SLC12A1 Rabbit Polyclonal Antibody
SKU:
CAB12999
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SLC12A1 (NP_000329.2).
This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven.
Purification Method:
Affinity purification
Gene ID:
6557
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SLC12A1 Rabbit pAb (CAB12999) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
