The SKP2 Rabbit Monoclonal Antibody (CAB4046) is a powerful tool for researchers studying SKP2, a protein involved in cell cycle regulation and cancer development. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human SKP2, making it ideal for Western blot applications.SKP2 is a key player in cell cycle progression, acting as a regulator of ubiquitin-mediated protein degradation. Dysregulation of SKP2 has been implicated in various cancers, making it an attractive target for cancer research. By targeting SKP2 with this monoclonal antibody, researchers can gain valuable insights into the role of SKP2 in cancer development and potentially identify new therapeutic targets.
This antibody provides a reliable tool for detecting and quantifying SKP2 protein levels in various cell types, facilitating further research into the mechanisms underlying SKP2's function in cancer biology. With its high specificity and sensitivity, the SKP2 Rabbit Monoclonal Antibody (CAB4046) is an essential reagent for studies aiming to unravel the complexity of SKP2-mediated pathways and their implications in cancer progression.
Product Name:
SKP2 Rabbit Monoclonal Antibody
SKU:
CAB4046
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SKP2 (Q13309).
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas. Alternative splicing of this gene generates three transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
6502
Clone Number:
ARC0886
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using SKP2 Rabbit mAb (CAB4046) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
