The SKP2 Polyclonal Antibody (CAB7728) is a valuable tool for researchers studying SKP2, a key protein involved in cell cycle regulation and cancer development. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting SKP2 in human samples, making it ideal for various research applications such as Western blotting.SKP2 is a crucial component of the SCF (SKP1-CUL1-F-Box protein) E3 ubiquitin ligase complex, responsible for targeting cell cycle regulators for degradation. Dysregulation of SKP2 has been associated with various cancers, making it a promising target for cancer therapy research.
By using the SKP2 Polyclonal Antibody (CAB7728), researchers can gain valuable insights into the expression levels and activity of SKP2 in different cell types and disease states.Understanding the role of SKP2 in cell cycle control and tumorigenesis is essential for developing targeted therapies that exploit the vulnerabilities of cancer cells and inhibit their growth. The SKP2 Polyclonal Antibody (CAB7728) provides researchers with a reliable tool to investigate the function of SKP2 and its potential as a therapeutic target in cancer treatment strategies.
Product Name:
SKP2 Rabbit Polyclonal Antibody
SKU:
CAB7728
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SKP2 (NP_005974.2).
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas. Alternative splicing of this gene generates three transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
6502
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SKP2 antibody (CAB7728) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
