The SKP1 Rabbit Monoclonal Antibody (CAB9021) is a highly specific antibody designed for research involving SKP1, a component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. This antibody, produced in rabbits, is recommended for use in Western blot and immunohistochemistry applications, allowing for the detection and analysis of SKP1 protein in various experimental settings.SKP1 is essential for the proper function of the SCF complex, which plays a crucial role in ubiquitin-mediated protein degradation. Aberrant regulation of this complex has been implicated in various diseases, including cancer and neurodegenerative disorders.
Therefore, studying the expression and activity of SKP1 using this specific antibody is essential for understanding the molecular mechanisms underlying these conditions.With its high reactivity and specificity towards human samples, the SKP1 Rabbit Monoclonal Antibody (CAB9021) is a valuable tool for researchers in the fields of cell biology, molecular biology, and cancer research. By enabling the precise detection of SKP1 protein, this antibody aids in advancing our knowledge of protein degradation pathways and potential therapeutic targets for disease intervention.
Product Name:
SKP1 Rabbit Monoclonal Antibody
SKU:
CAB9021
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 64-163 of human SKP1 (P63208).
This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.
Purification Method:
Affinity purification
Gene ID:
6500
Clone Number:
ARC1384
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using SKP1 Rabbit mAb (CAB9021) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
