The SKIV2L2 Polyclonal Antibody (CAB5576) is a valuable tool for researchers studying SKIV2L2, a protein involved in RNA processing and metabolism. This antibody, produced in rabbits, demonstrates high specificity and sensitivity towards human samples and has been validated for use in Western blot applications. By binding to the SKIV2L2 protein, this antibody allows for the detection and analysis of SKIV2L2 expression in various cell types, making it an essential tool for investigations in molecular biology and RNA biology.SKIV2L2 is a crucial component of the RNA exosome complex, which plays a vital role in degrading and processing RNA molecules within the cell.
Dysregulation of SKIV2L2 has been implicated in various diseases, including certain cancers and neurological disorders, highlighting its importance in cellular homeostasis. By studying the function and regulation of SKIV2L2, researchers can gain valuable insights into RNA metabolism and potentially identify new therapeutic targets for diseases associated with RNA processing abnormalities.
Product Name:
SKIV2L2 Rabbit Polyclonal Antibody
SKU:
CAB5576
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-304 of human SKIV2L2 (NP_056175.3).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
Dob1; Mtr4; SKIV2L2; fSAP118; KIAA0052
Positive Sample:
HeLa,22RV1,293T,U-87MG,Mouse testis,Mouse spleen
Conjugate:
Unconjugated
Cellular Localization:
Nucleus, nucleolus, nucleoplasm.
Calculated MW:
118kDa
Observed MW:
123kDa
Enables ATP binding activity and RNA helicase activity. Involved in RNA catabolic process; cellular response to DNA damage stimulus; and maturation of 5.8S rRNA. Located in nucleoplasm. Part of TRAMP complex and catalytic step 2 spliceosome. Colocalizes with nuclear exosome (RNase complex). Biomarker of amyotrophic lateral sclerosis.
Purification Method:
Affinity purification
Gene ID:
23517
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using SKIV2L2 Rabbit pAb (CAB5576) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
