SKA3 Antibody (PACO57316)
- SKU:
- PACO57316
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
SKA3 Antibody (PACO57316)
The SKA3 Polyclonal Antibody (PAC057316) is a valuable tool for researchers studying SKA3, a crucial component of the spindle and kinetochore complex involved in cell division. This antibody, generated in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications.SKA3 plays a vital role in the accurate segregation of chromosomes during mitosis, making it a key player in cell division and DNA replication processes. Dysregulation of SKA3 has been associated with chromosomal instability, a hallmark of cancer development. By targeting SKA3 with this specific antibody, researchers can analyze the protein expression and localization in various cell types, providing insights into its function and potential implications for cancer research.
The SKA3 Polyclonal Antibody is a valuable tool for understanding the role of SKA3 in cell division and its potential as a target for therapeutic interventions in cancer treatment. Its high specificity and reactivity make it an essential component in studies focusing on spindle and kinetochore complex dynamics, offering new avenues for research and discovery in the field of cell biology.
| Antibody Name: | SKA3 Antibody (PACO57316) |
| Antibody SKU: | PACO57316 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Spindle and kinetochore-associated protein 3 protein (101-195AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO57316 diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO57316 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO57316 diluted at 1:300 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization. Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules. |
| Synonyms: | Spindle and kinetochore-associated protein 3, SKA3, C13orf3 RAMA1 |
| UniProt Protein Function: | RAMA1: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization. Belongs to the SKA3 family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Unknown functionChromosomal Location of Human Ortholog: 13q12.11Cellular Component: outer kinetochore of condensed chromosome; spindle microtubuleMolecular Function: protein bindingBiological Process: chromosome segregation; mitosis; regulation of microtubule polymerization or depolymerization |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a component of the spindle and kinetochore-associated protein complex that regulates microtubule attachment to the kinetochores during mitosis. The encoded protein localizes to the outer kinetechore and may be required for normal chromosome segregation and cell division. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] |
| UniProt Code: | Q8IX90 |
| NCBI GenInfo Identifier: | 73620123 |
| NCBI Gene ID: | 221150 |
| NCBI Accession: | Q8IX90.2 |
| UniProt Secondary Accession: | Q8IX90,Q5VZV5, Q86WR2, Q8NBG1, Q96D22, A2A330, A2A331 B2RBY2 |
| UniProt Related Accession: | Q8IX90 |
| Molecular Weight: | 43,978 Da |
| NCBI Full Name: | Spindle and kinetochore-associated protein 3 |
| NCBI Synonym Full Names: | spindle and kinetochore associated complex subunit 3 |
| NCBI Official Symbol: | SKA3Â Â |
| NCBI Official Synonym Symbols: | RAMA1; C13orf3Â Â |
| NCBI Protein Information: | spindle and kinetochore-associated protein 3 |
| UniProt Protein Name: | Spindle and kinetochore-associated protein 3 |
| UniProt Synonym Protein Names: | |
| Protein Family: | Spindle and kinetochore-associated protein |
| UniProt Gene Name: | SKA3Â Â |
| UniProt Entry Name: | SKA3_HUMAN |
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