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SKA1 Antibody (PACO57372)

SKU:
PACO57372
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
IHC
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
€359

Description

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SKA1 Antibody (PACO57372)

The SKA1 Polyclonal Antibody (PAC05732) is a valuable tool for researchers studying the SKA1 protein, which is involved in cell division and plays a crucial role in maintaining chromosomal stability. This antibody, raised in rabbits, is highly specific to human SKA1 samples and has been validated for use in Western blot applications.SKA1, also known as spindle and kinetochore associated complex subunit 1, is essential for proper chromosome segregation during cell division. Dysregulation of SKA1 has been linked to genetic instability and cancer development, making it a key target for cancer research studies.

By using the SKA1 Polyclonal Antibody, researchers can detect and analyze levels of SKA1 in various cell types, allowing for a better understanding of its function in cell division and its potential implications in cancer biology. This antibody is a valuable tool for further elucidating the role of SKA1 in chromosomal stability and identifying potential therapeutic targets for cancer treatment.

Antibody Name:SKA1 Antibody (PACO57372)
Antibody SKU:PACO57372
Size:50ug
Host Species:Rabbit
Tested Applications:ELISA, IHC
Recommended Dilutions:ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:Human
Immunogen:Recombinant Human Spindle and kinetochore-associated protein 1 protein (61-134AA)
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:>95%, Protein G purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageIHC image of PACO57372 diluted at 1:200 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules. In the complex, it mediates the interaction with microtubules.
Synonyms:Spindle and kinetochore-associated protein 1, SKA1, C18orf24
UniProt Protein Function:SKA1: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules. Belongs to the SKA1 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Unknown functionChromosomal Location of Human Ortholog: 18q21.1Cellular Component: microtubule cytoskeleton; spindle microtubule; outer kinetochore of condensed chromosome; cytosolMolecular Function: protein binding; microtubule bindingBiological Process: mitosis; cell division; mitotic cell cycle; regulation of microtubule polymerization or depolymerization; chromosome segregation
UniProt Protein Details:
NCBI Summary:
UniProt Code:Q96BD8
NCBI GenInfo Identifier:74731226
NCBI Gene ID:220134
NCBI Accession:Q96BD8.1
UniProt Secondary Accession:Q96BD8,B2R9Y6, B4E0P4
UniProt Related Accession:Q96BD8
Molecular Weight:24,390 Da
NCBI Full Name:Spindle and kinetochore-associated protein 1
NCBI Synonym Full Names:spindle and kinetochore associated complex subunit 1
NCBI Official Symbol:SKA1  
NCBI Official Synonym Symbols:C18orf24  
NCBI Protein Information:spindle and kinetochore-associated protein 1; spindle and KT (kinetochore) associated 1
UniProt Protein Name:Spindle and kinetochore-associated protein 1
UniProt Synonym Protein Names:
Protein Family:Spindle and kinetochore-associated protein
UniProt Gene Name:SKA1  
UniProt Entry Name:SKA1_HUMAN
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