The SIRT5 Polyclonal Antibody (CAB5784) is a valuable tool for researchers studying SIRT5, a key protein involved in regulating cellular metabolism and stress response. Raised in rabbits, this antibody is highly specific for detecting SIRT5 in human samples and has been validated for use in Western blot applications. By binding to the SIRT5 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for research in the fields of metabolism, aging, and cancer biology.SIRT5 is a member of the sirtuin family of proteins, known for their roles in regulating various cellular processes such as DNA repair, gene expression, and metabolism. SIRT5 specifically plays a crucial role in modulating protein post-translational modifications, including lysine desuccinylation and demalonylation, which can impact cellular function and metabolism.
Understanding the function of SIRT5 is important for uncovering its potential implications in diseases such as cancer, metabolic disorders, and neurodegenerative conditions.Overall, the SIRT5 Polyclonal Antibody (CAB5784) is a valuable tool for researchers interested in studying the role of SIRT5 in cellular metabolism and disease pathways. Its high reactivity and specificity make it an essential component for experiments aimed at unraveling the complex functions of SIRT5 in health and disease.
Product Name:
[KO Validated] SIRT5 Rabbit Polyclonal Antibody
SKU:
CAB5784
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 37-310 of human SIRT5 (NP_036373.1).
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
23408
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and SIRT5 knockout (KO) 293T cells, using [KO Validated] SIRT5 Rabbit pAb (CAB5784) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
