The SIRT5 Polyclonal Antibody is a valuable tool for researchers studying SIRT5, a member of the sirtuin family of proteins known for their involvement in cellular metabolism and aging. This antibody, produced in rabbits, is highly specific for detecting SIRT5 in human samples and is validated for use in Western blot applications. By binding to the SIRT5 protein, this antibody allows for accurate detection and analysis in various cell types, making it ideal for research in the fields of metabolism, aging, and mitochondrial function.
SIRT5 has been linked to a variety of cellular processes, including the regulation of metabolism, DNA repair, and stress response pathways. Its role in these pathways makes it a key target for research into diseases such as cancer, metabolic disorders, and neurodegenerative conditions. By understanding the function of SIRT5, researchers can gain insights into the mechanisms underlying these diseases and potentially discover new targets for therapeutic intervention.
Product Name:
SIRT5 Polyclonal Antibody
SKU:
CAB21827
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 216-310 of human SIRT5 (NP_036373.1).
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
23408
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SIRT5 antibody (CAB21827) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
