Anti-SIRT3 Antibody (CAB5419)
- SKU:
- CAB5419
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
Anti-SIRT3 Antibody (CAB5419)
The Sirt3 Polyclonal Antibody (CAB5419) is a valuable tool for researchers investigating the role of Sirt3, a mitochondrial protein deacetylase implicated in various cellular processes including metabolism, stress response, and aging. This antibody, generated in rabbits, shows high specificity for human samples and is validated for use in Western blot applications. By binding to the Sirt3 protein, this antibody enables precise detection and quantification in a variety of cell types, making it an excellent choice for studies in biochemistry, cell biology, and aging research.
Sirt3 is known for its role in regulating mitochondrial function and metabolism, making it a promising target for therapeutic interventions in metabolic disorders, neurodegenerative diseases, and age-related conditions. Understanding the function and regulation of Sirt3 is crucial for developing strategies to manipulate its activity for beneficial outcomes. The Sirt3 Polyclonal Antibody offers researchers a reliable tool for studying the intricate role of Sirt3 in cellular processes and disease mechanisms.
| Antibody Name: | Anti-SIRT3 Antibody |
| Antibody SKU: | CAB5419 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-130 of human SIRT3 (NP_036371.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Mouse liver, Mouse kidney, Mouse brain, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-130 of human SIRT3 (NP_036371.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ERVE AGGG VGPF QACG CRLV LGGR DDVS AGLR GSHG ARGE PLDP ARPL QRPP RPEV PRAF RRQP RAAA PSFF FSSI KGGR RSIS FSVG ASSV VGSG GSSD KGKL SLQD VAE |
| Gene ID: | 23410 |
| Uniprot: | Q9NTG7 |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 28kDa/43kDa |
| Observed MW: | 30kDa |
| Synonyms: | SIRT3, SIR2L3, sirtuin 3 |
| Background: | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. |
| UniProt Code: | Q9NTG7 |
| NCBI GenInfo Identifier: | 38258651 |
| NCBI Gene ID: | 23410 |
| NCBI Accession: | Q9NTG7.2 |
| UniProt Secondary Accession: | Q9NTG7,Q9Y6E8, B7Z5U6, |
| UniProt Related Accession: | Q9NTG7 |
| Molecular Weight: | 399 |
| NCBI Full Name: | NAD-dependent protein deacetylase sirtuin-3, mitochondrial |
| NCBI Synonym Full Names: | sirtuin 3 |
| NCBI Official Symbol: | SIRT3Â Â |
| NCBI Official Synonym Symbols: | SIR2L3Â Â |
| NCBI Protein Information: | NAD-dependent protein deacetylase sirtuin-3, mitochondrial; sir2-like 3; sirtuin type 3; SIR2-like protein 3; regulatory protein SIR2 homolog 3; NAD-dependent deacetylase sirtuin-3, mitochondrial; silent mating type information regulation 2, S.cerevisiae, homolog 3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase |
| UniProt Protein Name: | NAD-dependent protein deacetylase sirtuin-3, mitochondrial |
| UniProt Synonym Protein Names: | Regulatory protein SIR2 homolog 3; SIR2-like protein 3 |
| Protein Family: | NAD-dependent protein deacetylase sirtuin |
| UniProt Gene Name: | SIRT3Â Â |
| UniProt Entry Name: | SIR3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SIRT3 antibody (CAB5419) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human prostate using SIRT3 antibody (CAB5419) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human esophagus using SIRT3 antibody (CAB5419) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using SIRT3 antibody (CAB5419) at dilution of 1:100 (40x lens). |
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