The SIRPA Polyclonal Antibody (CAB9001) is specifically designed for researchers studying the SIRPA protein, a cell surface molecule with important roles in immune regulation and cell signaling. This antibody, generated in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications.SIRPA, also known as signal regulatory protein alpha, is involved in the regulation of immune responses and phagocytosis, making it a key player in immune system function. By targeting the SIRPA protein, researchers can visualize and analyze its expression in various cell types, providing valuable insights into its role in immunology and potentially in diseases such as cancer and autoimmune disorders.
Understanding the function of SIRPA is essential for developing targeted therapies that modulate immune responses and potentially treat a variety of immune-related conditions. With its specificity and reliability, the SIRPA Polyclonal Antibody is a valuable tool for researchers exploring the complexities of the immune system and its impact on health and disease.
Product Name:
CD172a/SIRPα Rabbit Polyclonal Antibody
SKU:
CAB9001
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 220-375 of human CD172a/SIRPα (NP_542970.1).
The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene.
Purification Method:
Affinity purification
Gene ID:
140885
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CD172a/SIRPα antibody (CAB9001) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
