The Shroom3 Antibody (PAC046922) is a polyclonal antibody designed for research involving Shroom3, a protein that plays a key role in cell shape changes during tissue development and organ formation. This antibody, generated in rabbits, is highly specific and reactive with human samples, making it an ideal tool for studying the function and localization of Shroom3 in various cell types.Shroom3 is a critical regulator of actin cytoskeleton dynamics, influencing cell morphology and motility. Dysregulation of Shroom3 has been implicated in diseases such as cancer and developmental disorders.
By targeting Shroom3 with this antibody, researchers can investigate its involvement in these conditions and potentially identify new therapeutic targets.This antibody is validated for use in Western blot applications, allowing for the detection and analysis of Shroom3 protein levels. Its high specificity and sensitivity make it a valuable tool for studies in developmental biology, cancer research, and cell biology. By understanding the role of Shroom3 in cellular processes, researchers can gain insights into mechanisms of disease and potential interventions.
Immunohistochemistry of paraffin-embedded human endometrial cancer using PACO46922 at dilution of 1:100.
Immunofluorescent analysis of HepG2 cells using PACO46922 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Controls cell shape changes in the neuroepithelium during neural tube closure. Induces apical constriction in epithelial cells by promoting the apical accumulation of F-actin and myosin II, and probably by bundling stress fibers. Induces apicobasal cell elongation by redistributing gamma-tubulin and directing the assembly of robust apicobasal microtubule arrays (By similarity).
Synonyms:
Protein Shroom3 (Shroom-related protein) (hShrmL), SHROOM3, KIAA1481 SHRML
UniProt Protein Function:
SHRM: a filamentous-actin binding protein. Required for normal neural tube closure and cytoskeletal polarity in developing neuroepithelial cells.Protein type: CytoskeletalChromosomal Location of Human Ortholog: 4q21.1Cellular Component: adherens junction; apical junction complex; apical plasma membrane; cytoskeletonBiological Process: apical protein localization; cell morphogenesis; cellular pigment accumulation; pattern specification process
UniProt Protein Details:
NCBI Summary:
This gene encodes a PDZ-domain-containing protein that belongs to a family of Shroom-related proteins. This protein may be involved in regulating cell shape in certain tissues. A similar protein in mice is required for proper neurulation. [provided by RefSeq, Jan 2011]
