SHROOM2 Antibody (PACO57152)
- SKU:
- PACO57152
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
SHROOM2 Antibody (PACO57152)
The SHROOM2 Polyclonal Antibody (PAC057152) offered by AssayGenie is a valuable tool for researchers studying the SHROOM2 protein. SHROOM2 is a key regulator of cell shape and plays a crucial role in various cellular processes, including cell migration and tissue morphogenesis.This high-quality antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the SHROOM2 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for investigations in the fields of cell biology and developmental biology.
The SHROOM2 protein has been linked to various diseases and conditions, including cancer and developmental disorders. Understanding the function and regulation of SHROOM2 is essential for developing targeted therapies and advancing our knowledge of cellular processes.Overall, the SHROOM2 Polyclonal Antibody from AssayGenie offers researchers a reliable tool for exploring the role of SHROOM2 in health and disease, ultimately contributing to advancements in the biomedical sciences.
| Antibody Name: | SHROOM2 Antibody (PACO57152) |
| Antibody SKU: | PACO57152 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Protein Shroom2 protein (213-405AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunofluorescence staining of MCF-7 cells with PACO57152 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO57152 diluted at 1:200 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | May be involved in endothelial cell morphology changes during cell spreading. In the retinal pigment epithelium, may regulate the biogenesis of melanosomes and promote their association with the apical cell surface by inducing gamma-tubulin redistribution (By similarity). |
| Synonyms: | Protein Shroom2 (Apical-like protein) (Protein APXL), SHROOM2, APXL |
| UniProt Protein Function: | APXL: May be involved in endothelial cell morphology changes during cell spreading. In the retinal pigment epithelium, may regulate the biogenesis of melanosomes and promote their association with the apical cell surface by inducing gamma-tubulin redistribution. Belongs to the shroom family.Protein type: Channel, sodiumChromosomal Location of Human Ortholog: Xp22.3Cellular Component: apical plasma membrane; cell-cell adherens junction; cortical actin cytoskeleton; cytoskeleton; plasma membrane; tight junctionMolecular Function: actin binding; actin filament binding; amiloride-sensitive sodium channel activity; beta-catenin binding; protein bindingBiological Process: actin filament bundle formation; apical protein localization; brain development; camera-type eye development; camera-type eye morphogenesis; cell migration; cell morphogenesis; cellular pigment accumulation; ear development; establishment of melanosome localization; eye pigment granule organization and biogenesis; lens morphogenesis in camera-type eye; melanosome organization and biogenesis |
| UniProt Protein Details: | |
| NCBI Summary: | This gene represents the human homolog of Xenopus laevis apical protein (APX) gene, which is implicated in amiloride-sensitive sodium channel activity. It is expressed in endothelial cells and facilitates the formation of a contractile network within endothelial cells. Depletion of this gene results in an increase in endothelial sprouting, migration, and angiogenesis. This gene is highly expressed in the retina, and is a strong candidate for ocular albinism type 1 syndrome. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2016] |
| UniProt Code: | Q13796 |
| NCBI GenInfo Identifier: | 2498147 |
| NCBI Gene ID: | 357 |
| NCBI Accession: | Q13796.1 |
| UniProt Secondary Accession: | Q13796,B9EIQ7 |
| UniProt Related Accession: | Q13796 |
| Molecular Weight: | 176,410 Da |
| NCBI Full Name: | Protein Shroom2 |
| NCBI Synonym Full Names: | shroom family member 2 |
| NCBI Official Symbol: | SHROOM2Â Â |
| NCBI Official Synonym Symbols: | APXL; HSAPXLÂ Â |
| NCBI Protein Information: | protein Shroom2 |
| UniProt Protein Name: | Protein Shroom2 |
| UniProt Synonym Protein Names: | Apical-like protein; Protein APXL |
| Protein Family: | Protein |
| UniProt Gene Name: | SHROOM2Â Â |
| UniProt Entry Name: | SHRM2_HUMAN |
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