The SHPRH Polyclonal Antibody (PACO58144) is a valuable tool for research involving the SHPRH protein, a key player in DNA repair mechanisms. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the SHPRH protein, this antibody enables accurate detection and analysis in various cell types, making it an ideal choice for studies in molecular biology and cancer research.SHPRH, known for its role in DNA repair processes, is essential for maintaining genomic stability and preventing the accumulation of mutations that could lead to cancer development.
Understanding the function of SHPRH is crucial in unraveling the mechanisms of DNA repair and uncovering potential targets for cancer therapies. Research on SHPRH may provide valuable insights into the development of novel treatments for cancer and other genetic disorders.Overall, the SHPRH Polyclonal Antibody (PACO58144) offers researchers a reliable tool for investigating the biological functions of the SHPRH protein and its implications in DNA repair pathways, offering new avenues for exploring innovative therapeutic strategies.
IHC image of PACO58144 diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of SH-SY5Y cells with PACO58144 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'.
SHPRH: E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'. Belongs to the SNF2/RAD54 helicase family. 4 isoforms of the human protein are produced by alternative splicing.Protein type: EC 3.6.1.-; EC 3.6.4.-; EC 6.3.2.-; EC 6.3.2.19; Helicase; Ligase; Ubiquitin conjugating system; Ubiquitin ligaseChromosomal Location of Human Ortholog: 6q24.3Molecular Function: protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activityBiological Process: protein polyubiquitination; response to DNA damage stimulus
UniProt Protein Details:
NCBI Summary:
SHPRH is a ubiquitously expressed protein that contains motifs characteristics of several DNA repair proteins, transcription factors, and helicases. SHPRH is a functional homolog of S. cerevisiae RAD5 (Unk et al., 2006 [PubMed 17108083]).[supplied by OMIM, Mar 2008]
