The SHPRH Polyclonal Antibody (PAC017090) is a valuable tool for researchers studying SHPRH, a key protein involved in DNA repair and maintenance. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the SHPRH protein, researchers can accurately detect and analyze its expression in a variety of cell types, making it an essential asset for studies in genetics, cancer research, and molecular biology.SHPRH is known for its role in the DNA damage response, where it functions as a DNA translocase and plays a crucial part in maintaining genome stability.
Dysregulation of SHPRH has been implicated in various diseases, including cancer and neurodegenerative disorders. By investigating SHPRH activity and function, researchers can gain valuable insights into the underlying mechanisms of these conditions and potentially identify new therapeutic targets for intervention.Overall, the SHPRH Polyclonal Antibody offers researchers a powerful tool for exploring the functions and implications of SHPRH in health and disease. Its ability to specifically target the SHPRH protein makes it an invaluable resource for advancing our understanding of DNA repair mechanisms and their impact on human health.
Antibody Name:
SHPRH Antibody (PACO17090)
Antibody SKU:
PACO17090
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:30-1:150
Species Reactivity:
Human, Mouse
Immunogen:
Fusion protein of human SHPRH
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using PACO17090(SHPRH Antibody) at dilution 1/45, on the right is treated with fusion protein. (Original magnification: x200).
Background:
SHPRH is a ubiquitously expressed protein that contains motifs characteristics of several DNA repair proteins, transcription factors, and helicases. SHPRH is a functional homolog of S. cerevisiae RAD5 (Unk et al., 2006 [PubMed 17108083]).
Synonyms:
SNF2 histone linker PHD RING helicase, E3 ubiquitin protein ligase
UniProt Protein Function:
SHPRH: E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'. Belongs to the SNF2/RAD54 helicase family. 4 isoforms of the human protein are produced by alternative splicing.Protein type: EC 3.6.1.-; EC 3.6.4.-; EC 6.3.2.-; EC 6.3.2.19; Helicase; Ligase; Ubiquitin conjugating system; Ubiquitin ligaseChromosomal Location of Human Ortholog: 6q24.3Molecular Function: protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activityBiological Process: protein polyubiquitination; response to DNA damage stimulus
UniProt Protein Details:
NCBI Summary:
SHPRH is a ubiquitously expressed protein that contains motifs characteristics of several DNA repair proteins, transcription factors, and helicases. SHPRH is a functional homolog of S. cerevisiae RAD5 (Unk et al., 2006 [PubMed 17108083]).[supplied by OMIM, Mar 2008]
