The SHMT2 Polyclonal Antibody (CAB1215) is a crucial tool for researchers studying the SHMT2 protein, a vital enzyme involved in the one-carbon metabolism pathway. This antibody, generated in rabbits, exhibits high specificity towards human samples and is validated for use in Western blot applications. By binding to SHMT2, this antibody enables precise detection and analysis of the protein in a variety of cell types, making it well-suited for studies in metabolic regulation and cancer biology.SHMT2 plays a key role in cellular metabolism by facilitating the interconversion of serine and glycine, processes essential for nucleotide synthesis and cell proliferation.
Dysregulation of SHMT2 has been implicated in various diseases, including cancer and metabolic disorders, underscoring its importance as a therapeutic target. By unraveling the functions of SHMT2, researchers can gain valuable insights into disease mechanisms and develop innovative treatment strategies targeting one-carbon metabolism pathways.
Product Name:
[KO Validated] SHMT2 Rabbit Polyclonal Antibody
SKU:
CAB1215
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 265-504 of human SHMT2 (NP_005403.2).
This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
6472
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SHMT2 Rabbit pAb (CAB1215) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
