The SHMT1 Polyclonal Antibody is a vital tool for researchers studying SHMT1, a key enzyme involved in one-carbon metabolism and essential for cell growth and proliferation. This antibody, produced in rabbits, is highly specific for human samples and is validated for use in Western blot applications. By binding to the SHMT1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in cancer biology, metabolic disorders, and therapeutic development.SHMT1 is a critical enzyme in the folate metabolic pathway, playing a crucial role in nucleotide synthesis and DNA methylation.
Dysregulation of SHMT1 has been implicated in various diseases, including cancer, neural tube defects, and cardiovascular disorders. By characterizing the expression and activity of SHMT1, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets for precision medicine approaches.Overall, the SHMT1 Polyclonal Antibody is a valuable tool for researchers seeking to explore the role of SHMT1 in health and disease, with the potential to drive advancements in personalized medicine and therapeutic interventions.
Product Name:
SHMT1 Polyclonal Antibody
SKU:
CAB4113
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 285-444 of human SHMT1 (NP_683718.1).
This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
6470
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SHMT1 antibody (CAB4113) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
