The SHISA5 Polyclonal Antibody (PACO37914) is a vital tool for researchers studying the SHISA5 protein, a regulator of Wnt signaling pathways that play a crucial role in cellular growth and differentiation. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including immunofluorescence and immunohistochemistry.SHISA5, also known as CKAMP44, is a promising target for research in developmental biology and neuroscience due to its involvement in synaptic plasticity and neuronal communication. By binding to the SHISA5 protein, this antibody enables precise detection and analysis in different experimental settings, making it ideal for investigations into neurological disorders, cognitive function, and synaptic transmission.
Understanding the function of SHISA5 is essential for unraveling its role in synaptic plasticity and cognitive function, offering insights into potential therapeutic strategies for conditions such as epilepsy, Alzheimer's disease, and other neurological disorders. The SHISA5 Polyclonal Antibody is a valuable tool for researchers seeking to expand our understanding of Wnt signaling pathways and their impact on neural development and function.
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using PACO37914 at dilution of 1:100.
Immunoprecipitating SHISA5 in HepG2 whole cell lysate. Lane 1: Rabbit control IgG (1µg) instead of PACO37914 in HepG2 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PACO37914 (6µg) + HepG2 whole cell lysate (500µg). Lane 3: HepG2 whole cell lysate (10µg).
Immunofluorescence staining of A549 cells with PACO37914 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Can induce apoptosis in a caspase-dependent manner and plays a role in p53/TP53-dependent apoptosis.
Synonyms:
Protein shisa-5 (Putative NF-kappa-B-activating protein 120) (Scotin), SHISA5, SCOTIN
UniProt Protein Function:
SCOTIN: Can induce apoptosis in a caspase-dependent manner and plays a role in p53/TP53-dependent apoptosis. Belongs to the shisa family. 4 isoforms of the human protein are produced by alternative splicing.Protein type: Apoptosis; Membrane protein, integralChromosomal Location of Human Ortholog: 3p21.31Molecular Function: protein binding; signal transducer activityBiological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the shisa family. The encoded protein is localized to the endoplasmic reticulum, and together with p53 induces apoptosis in a caspase-dependent manner. Alternative splicing results in multiple transcript variants. Related pseudogenes of this gene are found on chromosome X. [provided by RefSeq, Apr 2016]
