Sonic Hedgehog (Shh) Rabbit Polyclonal Antibody (CAB12503)
The SHH Polyclonal Antibody (CAB12503) is a valuable tool for research involving the Sonic Hedgehog (SHH) signaling pathway. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it a reliable choice for studies in developmental biology, cancer research, and regenerative medicine.The SHH protein is a key player in embryonic development, where it regulates cell growth, differentiation, and patterning. Dysregulation of the SHH pathway has been linked to various types of cancer, making it an important target for therapeutic interventions.
The SHH Polyclonal Antibody binds specifically to the SHH protein, allowing for detection and analysis in a wide range of cell types and tissues.Understanding the role of SHH in various biological processes is essential for advancing our knowledge of developmental disorders and cancer progression. The SHH Polyclonal Antibody provides researchers with a reliable tool to investigate the intricate mechanisms of the SHH signaling pathway and its implications in health and disease.
Product Name:
Sonic Hedgehog (Shh) Rabbit Polyclonal Antibody
SKU:
CAB12503
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 231-330 of human Sonic Hedgehog (Shh) (NP_000184.1).
This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.
Purification Method:
Affinity purification
Gene ID:
6469
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of A-549 cells, using Sonic Hedgehog (Sonic Hedgehog (Shh)) antibody (CAB12503) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
