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Sheep Tumor Necrosis Factor Alpha (TNFa) ELISA Kit

SKU:
SHDL00005
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P23383
Sensitivity:
7.1pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
DIF,TNF-A,TNFSF2,Cachectin,Tumor Necrosis Factor Ligand Superfamily Member 2,TNF,Tumor Necrosis Factor
Reactivity:
Sheep
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Sheep Tumor Necrosis Factor Alpha (TNFa) ELISA Kit

The Sheep Tumor Necrosis Factor Alpha (TNFa) ELISA kit is specifically designed for the accurate detection of TNFa levels in sheep serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.TNFa is a key cytokine involved in inflammatory responses, immune regulation, and cell death. It plays a crucial role in various diseases such as cancer, autoimmune disorders, and infectious diseases.

By accurately measuring TNFa levels, researchers can gain valuable insights into disease progression and potential therapeutic targets.The Sheep TNFa ELISA kit provides researchers with a reliable tool for studying the role of TNFa in different biological processes and diseases. With its easy-to-use format and high-quality components, this kit is a valuable resource for researchers looking to advance their understanding of TNFa biology and its implications in disease pathology.

Product Name:Sheep Tumor Necrosis Factor Alpha (TNFa) ELISA Kit
Product Code:SHDL00005
Size:96 Assays
Target:TNFa
Synonyms:DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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