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Sheep Cytokine Array (18 targets) (SARB0092)

SKU:
SARB0092
Product Type:
Protein Array
Analytes:
AIF
Cadherin-6 (KCAD)
Decorin
IFN alpha
IFN-gamma
IL-1 alpha (IL-1 F1)
IL-1 beta (IL-1 F2)
IL-4
IL-13
IL-17A
IL-21
LEKTI (SPINK5)
LIF
MIG (CXCL9)
RANTES (CCL5)
sFRP-3
TNF alpha
VEGF-A
Reactivity:
Sheep
Applications:
Multiplex Array
€729 - €1,429

Description

system_update_altDatasheet

Sheep Cytokine Array (18 targets) (SARB0092)

The Sheep Cytokine Array (SARB0092) is a powerful research tool for studying the expression levels of 18 different cytokine targets in sheep samples. This array allows for simultaneous detection and quantification of multiple cytokines in a single experiment, providing valuable insights into immune responses and inflammatory processes in sheep.Each target on the array is carefully selected to cover a wide range of cytokine families, including interleukins, chemokines, and growth factors. By using this array, researchers can profile the cytokine expression patterns in various experimental conditions, such as infections, inflammatory diseases, and immune responses to vaccines.

The Sheep Cytokine Array is easy to use and provides reliable and reproducible results. It is suitable for a wide range of sample types, including serum, plasma, cell lysates, and tissue homogenates. With its high sensitivity and specificity, this array is an essential tool for studies in veterinary immunology, infectious diseases, and livestock health.Overall, the Sheep Cytokine Array (SARB0092) is a valuable resource for researchers interested in understanding the immune system of sheep and investigating the role of cytokines in health and disease in this important animal model.

Product Sku:SARB0092
Size:2, 4, or 8
Species Detected:Sheep
Number of Targets Detected:18
Gene Symbols:CCL5, DCN, FRZB, IFNG, IL-4, IL13, IL17A, IL1A, IL1B, IL21|IL4, LIF, SPINK5, TNF, TNFA, TNFSF2, VEGF, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
AIF
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #:
Cadherin-6 (KCAD)
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #:
Decorin
  • Gene Symbol: DCN
  • Gene ID: 443048
  • Accession #: Q9TTE2
IFN alpha
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #:
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 443396
  • Accession #: P17773
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: 443404
  • Accession #: Q28579
IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 443539
  • Accession #: P21621
IL-4
  • Gene Symbol: IL4 IL-4
  • Gene ID: 443327
  • Accession #: P30368
IL-13
  • Gene Symbol: IL13
  • Gene ID: 664707
  • Accession #: Q0PW91
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 101103931
  • Accession #: W5PWW6
IL-21
  • Gene Symbol: IL21
  • Gene ID: 100862657
  • Accession #: H6SGV5
LEKTI (SPINK5)
  • Gene Symbol: SPINK5
  • Gene ID: N/A
  • Accession #: W5P1K5
LIF
  • Gene Symbol: LIF
  • Gene ID: N/A
  • Accession #: W5P8L4
MIG (CXCL9)
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #:
RANTES (CCL5)
  • Gene Symbol: CCL5
  • Gene ID: N/A
  • Accession #: W5P4G7
sFRP-3
  • Gene Symbol: FRZB
  • Gene ID: 101118419
  • Accession #: W5Q5L6
TNF alpha
  • Gene Symbol: TNF, TNFA, TNFSF2
  • Gene ID: 443540
  • Accession #: P23383
VEGF-A
  • Gene Symbol: VEGFA, VEGF
  • Gene ID: 443103
  • Accession #: P50412
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Sheep Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis

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