The SHC1 Polyclonal Antibody (CAB7725) is a powerful tool for researchers studying SHC1, a critical protein involved in cell signaling pathways and regulation. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the SHC1 protein, this antibody allows for reliable detection and analysis in various cell types, making it ideal for investigations in molecular biology, cell signaling, and cancer research.SHC1 is a key adaptor protein that plays a pivotal role in transmitting extracellular signals to the cell interior, influencing processes such as cell growth, differentiation, and survival.
Dysregulation of SHC1 signaling has been implicated in various diseases, including cancer, diabetes, and cardiovascular disorders. Therefore, studying the function and regulation of SHC1 is essential for unraveling the underlying mechanisms of these conditions and identifying potential therapeutic targets.By utilizing the SHC1 Polyclonal Antibody (CAB7725), researchers can gain valuable insights into the role of SHC1 in cellular signaling pathways and its potential implications in disease pathogenesis. This antibody is a reliable tool for furthering our understanding of SHC1 biology and exploring its therapeutic potential in various pathological conditions.
Product Name:
SHC1 Rabbit Polyclonal Antibody
SKU:
CAB7725
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human SHC1 (NP_001189788.1).
This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6464
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using SHC1 Rabbit pAb (CAB7725) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
