Anti-SH2B2 Antibody (PACO19320)

Antibody Name:	SH2B2 Antibody (PACO19320)
Antibody SKU:	PACO19320
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, IHC
Recommended Dilutions:	ELISA:1:1000-1:2000, IHC:1:25-1:100
Species Reactivity:	Human, Rat
Immunogen:	Synthetic peptide of human SH2B2

Form:	Liquid
Storage Buffer:	-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:	Antigen affinity purification
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO19320(SH2B2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200).
	The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using PACO19320(SH2B2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200).

Background:

The protein encoded by this gene is expressed in B lymphocytes and contains pleckstrin homology and src homology 2 (SH2) domains. In Burkitt's lymphoma cell lines, it is tyrosine-phosphorylated in response to B cell receptor stimulation. Because it binds Shc independent of stimulation and Grb2 after stimulation, it appears to play a role in signal transduction from the receptor to the Shc/Grb2 pathway.

Synonyms:

SH2B adaptor protein 2

UniProt Protein Function:	APS: an adapter protein involved in coupling immunoreceptors to Ras signaling. Acts as a negative regulator of cytokine signaling in collaboration with CBL. Binds to EPOR and suppresses EPO-induced STAT5 activation, possibly through a masking effect on STAT5 docking sites in EPOR. Suppresses PDGF-induced mitogenesis. Interacts with several proteins including KIT/c-KIT, SHC, GRB2, EPOR, CBL/c-CBL, PDGFR, VAV1 and VAV3. Interacts with SHC through its N-terminal region, with GRB2 via the phosphorylated C-terminus and with EPOR and KIT via its SH2 domain. Interacts with GRB2 after B-cell antigen receptor stimulation. Interacts with VAV3 via its PH domain, inducing cytoskeletal reorganization. Expressed in spleen, prostrate, testis, uterus, small intestine and skeletal muscle. Among haematopoietic cell lines, expressed exclusively in B-cells. Not expressed in most tumor cell lines. Tyrosine phosphorylated by JAK2, KIT and other kinases activated by B-cell receptor in response to stimulation with cytokines, IL3, IL5, PDGF, IGF1, IGF2, CSF2/GM-CSF and cross-linking of the B-cell receptor complex.Protein type: Adaptor/scaffoldChromosomal Location of Human Ortholog: 7q22Cellular Component: ruffle; cytoplasm; plasma membrane; stress fiber; actin filament; cytosolMolecular Function: identical protein binding; protein binding; signal transducer activity; SH3/SH2 adaptor activity; transmembrane receptor protein tyrosine kinase adaptor protein activity; JAK pathway signal transduction adaptor activityBiological Process: regulation of Ras protein signal transduction; antigen receptor-mediated signaling pathway; nervous system development; positive regulation of signal transduction; cytokine and chemokine mediated signaling pathway; B-1 B cell homeostasis; signal transduction; regulation of JAK-STAT cascade; brown fat cell differentiation; insulin receptor signaling pathway; regulation of metabolic process; actin cytoskeleton organization and biogenesis; blood coagulation
UniProt Protein Details:
NCBI Summary:	The protein encoded by this gene is expressed in B lymphocytes and contains pleckstrin homology and src homology 2 (SH2) domains. In Burkitt's lymphoma cell lines, it is tyrosine-phosphorylated in response to B cell receptor stimulation. Because it binds Shc independent of stimulation and Grb2 after stimulation, it appears to play a role in signal transduction from the receptor to the Shc/Grb2 pathway. [provided by RefSeq, Jun 2009]
UniProt Code:	O14492
NCBI GenInfo Identifier:	206729900
NCBI Gene ID:	10603
NCBI Accession:	O14492.2
UniProt Secondary Accession:	O14492,A6ND74
UniProt Related Accession:	O14492
Molecular Weight:	67,738 Da
NCBI Full Name:	SH2B adapter protein 2
NCBI Synonym Full Names:	SH2B adaptor protein 2
NCBI Official Symbol:	SH2B2
NCBI Official Synonym Symbols:	APS
NCBI Protein Information:	SH2B adapter protein 2; SH2 and PH domain-containing adapter protein APS; adapter protein with pleckstrin homology and Src homology 2 domains
UniProt Protein Name:	SH2B adapter protein 2
UniProt Synonym Protein Names:	Adapter protein with pleckstrin homology and Src homology 2 domains; SH2 and PH domain-containing adapter protein APS
Protein Family:	SH2B adapter protein
UniProt Gene Name:	SH2B2
UniProt Entry Name:	SH2B2_HUMAN

Antibodies

SH2B2 Antibody (PACO02342)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)