The SGOL1 Polyclonal Antibody (CAB16174) is a valuable tool for researchers studying SGOL1, a key protein involved in cell division and chromosome segregation. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the SGOL1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it essential for studies in molecular biology and cancer research.SGOL1, also known as shugoshin-like 1, plays a crucial role in ensuring the accurate distribution of chromosomes during cell division.
Dysregulation of SGOL1 has been implicated in various diseases, including cancer and developmental disorders. By studying the function and expression of SGOL1, researchers can gain valuable insights into mechanisms of tumorigenesis and potential therapeutic targets for cancer treatment.Overall, the SGOL1 Polyclonal Antibody (CAB16174) is a reliable tool for investigating the role of SGOL1 in cellular processes and disease pathology. Its high specificity and sensitivity make it an indispensable asset for researchers working in the fields of cell biology and oncology.
Product Name:
SGOL1 Rabbit Polyclonal Antibody
SKU:
CAB16174
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 10-100 of human SGOL1 (NP_612493.1).
Chromosome, Cytoplasm, Nucleus, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, spindle pole
Calculated MW:
64kDa
Observed MW:
75kDa
The protein encoded by this gene is a member of the shugoshin family of proteins. This protein is thought to protect centromeric cohesin from cleavage during mitotic prophase by preventing phosphorylation of a cohesin subunit. Reduced expression of this gene leads to the premature loss of centromeric cohesion, mis-segregation of sister chromatids, and mitotic arrest. Evidence suggests that this protein also protects a small subset of cohesin found along the length of the chromosome arms during mitotic prophase. An isoform lacking exon 6 has been shown to play a role in the cohesion of centrioles (PMID: 16582621 and PMID:18331714). Mutations in this gene have been associated with Chronic Atrial and Intestinal Dysrhythmia (CAID) syndrome, characterized by the co-occurrence of Sick Sinus Syndrome (SSS) and Chronic Intestinal Pseudo-obstruction (CIPO) within the first four decades of life (PMID:25282101). Fibroblast cells from CAID patients exhibited both increased cell proliferation and higher rates of senescence. Pseudogenes of this gene have been found on chromosomes 1 and 7. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
151648
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SGOL1 Rabbit pAb (CAB16174) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
