SGMS2 Antibody (PACO39958)
- SKU:
- PACO39958
- Product Type:
- Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
SGMS2 Antibody (PACO39958)
The SGMS2 Polyclonal Antibody (PAC039958) is a valuable tool for researchers studying the SGMS2 protein, which is involved in sphingomyelin metabolism and cell signaling pathways. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the SGMS2 protein, this antibody allows for the detection and analysis of SGMS2 in a variety of cell types, making it ideal for studies in lipid metabolism, cell biology, and cancer research.SGMS2, also known as sphingomyelin synthase 2, plays a crucial role in the biosynthesis of sphingomyelin, a major component of cell membranes.
Dysregulation of SGMS2 has been implicated in various human diseases, including cancer, neurodegenerative disorders, and metabolic syndromes. By understanding the function and regulation of SGMS2, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets for intervention. The SGMS2 Polyclonal Antibody is a valuable tool for investigating the role of SGMS2 in health and disease.
| Antibody Name: | SGMS2 Antibody (PACO39958) |
| Antibody SKU: | PACO39958 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500 |
| Species Reactivity: | Human, Rat |
| Immunogen: | Recombinant Human Phosphatidylcholine:ceramide cholinephosphotransferase 2 protein (1-79AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Rat liver tissue. All lanes: SGMS2 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43 kDa. Observed band size: 43 kDa. |
 | Immunofluorescence staining of Hela cells with PACO39958 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO39958 diluted at 1:600 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Plasma membrane SMS2 can also convert phosphatidylethanolamine (PE) to ceramide phosphatidylethanolamine (CPE). Major form in liver. Required for cell growth in certain cell types. Regulator of cell surface levels of ceramide, an important mediator of signal transduction and apoptosis. Regulation of sphingomyelin (SM) levels at the cell surface affects insulin sensitivity. |
| Synonyms: | Phosphatidylcholine:ceramide cholinephosphotransferase 2 (EC 2.7.8.27) (Sphingomyelin synthase 2), SGMS2, SMS2 |
| UniProt Protein Function: | SMS2: Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Plasma membrane SMS2 can also convert phosphatidylethanolamine (PE) to ceramide phosphatidylethanolamine (CPE). Major form in liver. Required for cell growth in certain cell types. Regulator of cell surface levels of ceramide, an important mediator of signal transduction and apoptosis. Regulation of sphingomyelin (SM) levels at the cell surface affects insulin sensitivity. Belongs to the sphingomyelin synthase family.Protein type: Transferase; EC 2.7.8.27; Membrane protein, multi-pass; Membrane protein, integral; Lipid Metabolism - sphingolipidChromosomal Location of Human Ortholog: 4q25Cellular Component: Golgi apparatus; integral to Golgi membrane; integral to plasma membrane; plasma membraneMolecular Function: ceramide cholinephosphotransferase activity; sphingomyelin synthase activityBiological Process: sphingolipid biosynthetic process; sphingomyelin biosynthetic process |
| UniProt Protein Details: | |
| NCBI Summary: | Sphingomyelin, a major component of cell and Golgi membranes, is made by the transfer of phosphocholine from phosphatidylcholine onto ceramide, with diacylglycerol as a side product. The protein encoded by this gene is an enzyme that catalyzes this reaction primarily at the cell membrane. The synthesis is reversible, and this enzyme can catalyze the reaction in either direction. The encoded protein is required for cell growth. Three transcript variants encoding the same protein have been found for this gene. There is evidence for more variants, but the full-length nature of their transcripts has not been determined.[provided by RefSeq, Oct 2008] |
| UniProt Code: | Q8NHU3 |
| NCBI GenInfo Identifier: | 44888519 |
| NCBI Gene ID: | 166929 |
| NCBI Accession: | Q8NHU3.1 |
| UniProt Secondary Accession: | Q8NHU3,A8K2S9, B2RA61 |
| UniProt Related Accession: | Q8NHU3 |
| Molecular Weight: | 42,280 Da |
| NCBI Full Name: | Phosphatidylcholine:ceramide cholinephosphotransferase 2 |
| NCBI Synonym Full Names: | sphingomyelin synthase 2 |
| NCBI Official Symbol: | SGMS2Â Â |
| NCBI Official Synonym Symbols: | SMS2Â Â |
| NCBI Protein Information: | phosphatidylcholine:ceramide cholinephosphotransferase 2 |
| UniProt Protein Name: | Phosphatidylcholine:ceramide cholinephosphotransferase 2 |
| UniProt Synonym Protein Names: | Sphingomyelin synthase 2 |
| Protein Family: | |
| UniProt Gene Name: | SGMS2Â Â |
| UniProt Entry Name: | SMS2_HUMAN |
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