The SGCA Polyclonal Antibody (CAB4109) is a key tool for researchers studying SGCA, a protein found in striated muscle cells that is crucial for muscle structure and function. This antibody, produced in rabbits, is highly specific to the human SGCA protein and has been validated for use in Western blotting applications. By targeting the SGCA protein, researchers can visualize and analyze its expression in various cell types, making it ideal for studies in muscular dystrophy, muscle development, and muscle regeneration research.
SGCA is a critical component of the dystrophin-glycoprotein complex, essential for maintaining muscle integrity and stability. Mutations in the SGCA gene have been linked to several forms of muscular dystrophy, highlighting its importance in muscle health. Understanding the function and regulation of SGCA can provide insights into muscle disease mechanisms and aid in the development of potential therapeutic strategies for muscle disorders.
Recombinant fusion protein containing a sequence corresponding to amino acids 60-290 of human alpha Sarcoglycan (alpha Sarcoglycan (SGCA)) (NP_000014.1).
Cell membrane, Cytoplasm, Single-pass type I membrane protein, cytoskeleton, sarcolemma.
Calculated MW:
43kDa
Observed MW:
43kDa
This gene encodes a component of the dystrophin-glycoprotein complex (DGC), which is critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Its expression is thought to be restricted to striated muscle. Mutations in this gene result in type 2D autosomal recessive limb-girdle muscular dystrophy. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6442
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using alpha Sarcoglycan (SGCA) Rabbit pAb (CAB4109) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
