The SF3B5 Polyclonal Antibody (CAB15905) is designed for research involving SF3B5, a key component of the spliceosome complex essential for pre-mRNA splicing. The antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. It specifically binds to the SF3B5 protein, allowing for accurate detection and analysis in a variety of cell types, making it ideal for studies in molecular biology and RNA splicing mechanisms.
SF3B5 plays a crucial role in the spliceosome complex, which is responsible for accurately removing introns from pre-mRNA to generate mature mRNA. Dysregulation of SF3B5 has been linked to various diseases, including cancer and genetic disorders, highlighting its importance in RNA processing and gene expression. Further research on SF3B5 is essential for understanding its role in disease pathology and identifying potential therapeutic targets for intervention.
Product Name:
SF3B5 Rabbit Polyclonal Antibody
SKU:
CAB15905
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-86 of human SF3B5 (NP_112577.1).
Enables RNA binding activity and splicing factor binding activity. Involved in mRNA splicing, via spliceosome. Located in nucleoplasm. Part of U12-type spliceosomal complex and U2-type precatalytic spliceosome.
Purification Method:
Affinity purification
Gene ID:
83443
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SF3B5 Rabbit pAb (CAB15905) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
