The SF3B3 Rabbit Monoclonal Antibody (CAB9624) is a research tool specifically designed for the study of SF3B3, a key component of the spliceosome complex involved in RNA splicing. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the SF3B3 protein, researchers can accurately detect and analyze its expression in various cell types, making it an excellent choice for investigations in molecular biology and gene regulation.
SF3B3 is essential for proper RNA splicing, a critical process in gene expression that can impact cellular function and contribute to disease development. By uncovering the role of SF3B3, researchers can gain valuable insights into mechanisms of RNA processing and potentially identify novel therapeutic targets for conditions related to aberrant splicing, such as cancer and genetic disorders. This antibody offers a valuable tool for unraveling the complex functions of SF3B3 and advancing our understanding of RNA biology.
Product Name:
SF3B3/SAP130 Rabbit Monoclonal Antibody
SKU:
CAB9624
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1101-1217 of human SF3B3/SAP130 (Q15393).
This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.
Purification Method:
Affinity purification
Gene ID:
23450
Clone Number:
ARC1667
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using SF3B3/SAP130 Rabbit mAb (CAB9624) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
