The SF3B2 Polyclonal Antibody (CAB5875) is specifically designed for researchers studying SF3B2, a core component of the spliceosome complex involved in RNA splicing. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the SF3B2 protein, this antibody enables precise detection and analysis in diverse cell types, making it an invaluable tool for investigations in molecular biology and gene expression studies.
SF3B2 plays a crucial role in pre-mRNA splicing, where it is involved in the recognition and removal of introns from precursor messenger RNA. Dysregulation of SF3B2 has been associated with various cancers and genetic disorders, highlighting its importance in cellular processes. Research on SF3B2 is essential for understanding the mechanisms of RNA splicing and its implications in disease development, paving the way for potential therapeutic interventions targeting this pathway.
Product Name:
SF3B2 Rabbit Polyclonal Antibody
SKU:
CAB5875
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 616-895 of human SF3B2 (NP_006833.2).
This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.
Purification Method:
Affinity purification
Gene ID:
10992
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using SF3B2 Rabbit pAb (CAB5875) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
