The SF3B1 Polyclonal Antibody (CAB15801) is a valuable tool for researchers studying SF3B1, a key component of the spliceosome complex involved in RNA splicing. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications.SF3B1 plays a crucial role in maintaining the integrity of the spliceosome machinery, ensuring accurate splicing of pre-mRNA molecules. Dysregulation of SF3B1 has been linked to various diseases, including cancer and genetic disorders, making it an important target for research in molecular biology and oncology.
By specifically binding to SF3B1 protein, this polyclonal antibody enables detection and analysis of SF3B1 expression in different cell types, providing valuable insights into its function and potential role in disease progression. Researchers can utilize this antibody to advance their understanding of RNA splicing mechanisms and explore therapeutic strategies targeting SF3B1 in the treatment of diseases associated with splicing defects.
Product Name:
SF3B1 Rabbit Polyclonal Antibody
SKU:
CAB15801
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human SF3B1 (NP_001005526.1).
This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
23451
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SF3B1 Rabbit pAb (CAB15801) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
