Anti-Septin-9 Antibody (CAB8657)

Antibody Name:	Anti-Septin-9 Antibody
Antibody SKU:	CAB8657
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human SEPT9 (NP_001106964.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, HeLa, 293T, U-87MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human SEPT9 (NP_001106964.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEPP ASKV PEVP TAPA TDAA PKRV EIQM PKPA EAPT APSP AQTL ENSE PAPV SQLQ SRLE PKPQ PPVA EATP RSQE ATEA APSC VGDM ADTP RDAG LKQA PASR NEKA PV
Gene ID:	10801
Uniprot:	Q9UHD8
Cellular Location:	Cytoplasm, cytoskeleton
Calculated MW:	38kDa/41kDa/47kDa/52kDa/63kDa/64kDa/65kDa
Observed MW:	75kDa

Synonyms:

SEPT9, AF17q25, MSF, MSF1, NAPB, PNUTL4, SINT1, SeptD1, septin-9

Background:

This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.

UniProt Protein Function:	Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.
NCBI Summary:	This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2009]
UniProt Code:	Q9UHD8
NCBI GenInfo Identifier:	18203688
NCBI Gene ID:	10801
NCBI Accession:	AAH21192.1
UniProt Secondary Accession:	Q9UHD8,Q96QF3, Q96QF4, Q96QF5, Q9HA04, Q9UG40, A8K2V3 B3KPM0, B4DTL9, B4E0N2, B4E274, B7Z654,
UniProt Related Accession:	Q9UHD8
Molecular Weight:	41,187 Da
NCBI Full Name:	Septin 9
NCBI Synonym Full Names:	septin 9
NCBI Official Symbol:	SEPT9
NCBI Official Synonym Symbols:	MSF; MSF1; NAPB; SINT1; PNUTL4; SeptD1; AF17q25
NCBI Protein Information:	septin-9
UniProt Protein Name:	Septin-9
UniProt Synonym Protein Names:	MLL septin-like fusion protein MSF-A; MLL septin-like fusion protein; Ovarian/Breast septin; Ov/Br septin; Septin D1
UniProt Gene Name:	SEPT9
UniProt Entry Name:	SEPT9_HUMAN

	Western blot analysis of extracts of various cell lines, using SEPT9 antibody (CAB8657) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Confocal immunofluorescence analysis of U2OS cells using SEPT9 Polyclonal Antibody (CAB8657) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)