The SENP6 Polyclonal Antibody (CAB17673) is a valuable tool for research involving SENP6, a protein involved in the regulation of SUMOylation, a post-translational modification process that regulates various cellular functions. The antibody, derived from rabbit sources, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the SENP6 protein, this antibody enables the detection and analysis of SENP6 levels in different cell types, making it an ideal choice for studies in cell biology and molecular biology.SENP6 plays a crucial role in the regulation of SUMOylation, a process that involves the attachment of Small Ubiquitin-like Modifier (SUMO) proteins to target substrates to modulate their function.
Dysregulation of SUMOylation has been implicated in various diseases, including cancer, neurodegenerative disorders, and viral infections. By investigating the role of SENP6 in this process, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets. The SENP6 Polyclonal Antibody is a valuable tool for furthering our understanding of SUMOylation regulation and its implications in human health and disease.
Product Name:
SENP6 Rabbit Polyclonal Antibody
SKU:
CAB17673
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 811-1112 of human SENP6 (NP_056386.2).
Ubiquitin-like molecules (UBLs), such as SUMO1 (UBL1; MIM 601912), are structurally related to ubiquitin (MIM 191339) and can be ligated to target proteins in a similar manner as ubiquitin. However, covalent attachment of UBLs does not result in degradation of the modified proteins. SUMO1 modification is implicated in the targeting of RANGAP1 (MIM 602362) to the nuclear pore complex, as well as in stabilization of I-kappa-B-alpha (NFKBIA; MIM 164008) from degradation by the 26S proteasome. Like ubiquitin, UBLs are synthesized as precursor proteins, with 1 or more amino acids following the C-terminal glycine-glycine residues of the mature UBL protein. Thus, the tail sequences of the UBL precursors need to be removed by UBL-specific proteases, such as SENP6, prior to their conjugation to target proteins (Kim et al., 2000 [PubMed 10799485]). SENPs also display isopeptidase activity for deconjugation of SUMO-conjugated substrates (Lima and Reverter, 2008 [PubMed 18799455]).
Purification Method:
Affinity purification
Gene ID:
26054
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SENP6 Rabbit pAb (CAB17673) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
