The SENP5 Antibody (PAC017070) is a powerful tool for researchers studying SENP5, a protein involved in the regulation of various cellular processes. This polyclonal antibody is raised in rabbits and exhibits high reactivity with human samples, making it suitable for use in a wide range of research applications. Validated for Western blotting, the SENP5 Antibody specifically binds to the SENP5 protein, allowing for accurate detection and analysis in different cell types.SENP5, also known as Sentrin/SUMO-specific protease 5, plays a crucial role in the SUMOylation pathway and is involved in the regulation of gene expression, DNA repair, and cellular signaling.
Dysregulation of SENP5 has been implicated in various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions. By studying the function of SENP5, researchers can gain important insights into the underlying mechanisms of these diseases and potentially identify novel therapeutic targets.The SENP5 Antibody is a valuable tool for researchers interested in understanding the role of SENP5 in cellular processes and disease pathology. Its high specificity and sensitivity make it an ideal choice for studies in molecular biology, cancer research, and other fields where SENP5 is a key focus of investigation.
Antibody Name:
SENP5 Antibody (PACO17070)
Antibody SKU:
PACO17070
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:5000, WB:1:500-1:2000
Species Reactivity:
Human, Mouse
Immunogen:
Fusion protein of human SENP5
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Hela cells, K562 cells, NIH/3T3 cells, Primary antibody: PACO17070(SENP5 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds.
Background:
The reversible posttranslational modification of proteins by the addition of small ubiquitin-like SUMO proteins (see SUMO1; MIM 601912) is required for numerous biologic processes. SUMO-specific proteases, such as SENP5, are responsible for the initial processing of SUMO precursors to generate a C-terminal diglycine motif required for the conjugation reaction. They also have isopeptidase activity for the removal of SUMO from high molecular mass SUMO conjugates.
Synonyms:
SUMO1/sentrin specific peptidase 5
UniProt Protein Function:
SENP5: Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. Has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. Required for cell division. Belongs to the peptidase C48 family.
Molecular Function:endopeptidase activity; protein binding; SUMO-specific protease activity
Biological Process: protein desumoylation; protein sumoylation
NCBI Summary:
The reversible posttranslational modification of proteins by the addition of small ubiquitin-like SUMO proteins (see SUMO1; MIM 601912) is required for numerous biologic processes. SUMO-specific proteases, such as SENP5, are responsible for the initial processing of SUMO precursors to generate a C-terminal diglycine motif required for the conjugation reaction. They also have isopeptidase activity for the removal of SUMO from high molecular mass SUMO conjugates (Di Bacco et al., 2006 [PubMed 16738315]).[supplied by OMIM, Jun 2009]
