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Senescence Marker ELISA Sampler Pack

SKU:
SKFI0021
Product Type:
ELISA Kit
ELISA Type:
ELISA Sampler Pack
Size:
6 X 48 Assays
Reactivity:
Human
Analytes:
IL-6
CDKN2A
LMNB1
MMP3
HMGB1
TNF Alpha
  • Human IL-6 ELISA Kit
  • Human TNF alpha ELISA Kit
  • Human HMGB1 ELISA Kit
  • Human LMNB1 / Lamin B1 ELISA Kit
  • Human MMP3 ELISA Kit
  • Human CDKN2A / p16INK4a ELISA Kit
€1,650
Frequently bought together:

Description

Senescence Marker ELISA Sampler Kit

The Senescence Marker ELISA Kit Pack is a comprehensive set of ELISA kits designed for the accurate measurement of senescence markers in various biological samples. This pack includes ELISA kits for the detection of key senescence markers such as p16, p21, and SA-β-Gal, providing researchers with a complete solution for studying cellular senescence.Senescence is a vital process involved in aging, cancer, and other age-related diseases. By accurately measuring senescence markers, researchers can gain insights into the mechanisms underlying these conditions and develop targeted therapies.

The Senescence Marker ELISA Kit Pack offers high sensitivity and specificity, ensuring reliable and reproducible results for a wide range of research applications.With the Senescence Marker ELISA Kit Pack, researchers can explore the role of senescence markers in various biological processes and diseases, advancing our understanding of aging and age-related conditions. Order your kit today and start unraveling the mysteries of cellular senescence.

Senescence Marker ELISA Sampler Pack

Senescence Marker ELISA Sampler Pack from Assay Genie allows researchers to begin their investigation into Senescence Marker via analysing IL-6, CDKN2A / p16INK4a, LMNB1 / Lamin B1, MMP3, HMGB1, TNF alpha in a 48 assay format, thus allowing researchers to identify and validate analytes within the pathway.

Pack ContentsSizeReactivitySensitivityRange ELISA Type
Human IL-6 ELISA Kit48 AssaysHuman2.813pg/ml4.688-300pg/mlSandwich ELISA
Human CDKN2A / p16INK4a ELISA Kit48 AssaysHuman0.375ng/ml0.625-40ng/mlSandwich ELISA
Human LMNB1 / Lamin B1 ELISA Kit48 AssaysHuman0.094ng/ml0.156-10ng/mlSandwich ELISA
Human MMP3 ELISA Kit48 AssaysHuman0.094ng/ml0.156-10ng/mlSandwich ELISA
Human HMGB1 ELISA Kit48 AssaysHuman18.75pg/ml31.25-2000pg/mlSandwich ELISA
Human TNF alpha ELISA Kit48 AssaysHuman9.375pg/ml15.625-1000pg/mlSandwich ELISA

Each individual ELISA kit in this pack will come with the following components:

Component Quantity Storage
ELISA Microplate (Dismountable) 4-12 strips 4°C for 6 months
Lyophilized Standard 1 4°C/-20°C
Sample/Standard Dilution Buffer 10ml 4°C
Biotin-labeled Antibody(Concentrated) 60ul 4°C (Protect from light)
Antibody Dilution Buffer 5ml 4°C
HRP-Streptavidin Conjugate(SABC) 60ul 4°C (Protect from light)
SABC Dilution Buffer 5ml 4°C
TMB Substrate 10ml 4°C (Protect from light)
Stop Solution 5ml 4°C
Wash Buffer(25X) 15ml 4°C
Plate Sealer 3 -

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample Type Protocol
Serum If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.
Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 - g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal Fluid Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatant Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysates Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenates The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysates Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast Milk Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

StepProtocol
1.Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3.Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6.Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9.Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11.Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

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