The SEMA4B Polyclonal Antibody (PACO56170) is a valuable tool for researchers studying SEMA4B, a protein involved in cell signaling and immune regulation. This antibody, developed in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the SEMA4B protein, this antibody enables accurate detection and analysis in various cell types, making it an essential component for studies in immunology and cancer research.SEMA4B is a membrane-associated protein that plays a crucial role in modulating immune responses and cell communication. It is known to regulate cellular processes such as proliferation, migration, and differentiation, making it a key target for investigations into diseases like cancer and inflammatory conditions.
Understanding the function of SEMA4B is essential for developing targeted therapies that can manipulate immune responses and potentially treat a variety of conditions.Overall, the SEMA4B Polyclonal Antibody (PACO56170) provides researchers with a powerful tool for studying the role of SEMA4B in immune regulation and disease progression. Its high specificity and sensitivity make it a reliable option for experiments aimed at elucidating the mechanisms underlying immune responses and cell signaling pathways.
Western Blot. Positive WB detected in: Rat luing tissue. All lanes: SEMA4B antibody at 6.3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 93, 82 kDa. Observed band size: 93 kDa.
IHC image of PACO56170 diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO56170 diluted at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
Synonyms:
Semaphorin-4B, SEMA4B, KIAA1745 SEMAC
UniProt Protein Function:
SEMA4B iso3: a single-pass type I membrane protein that inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Two alternatively spliced human isoforms have been described.Protein type: Cell surface; Membrane protein, integralChromosomal Location of Human Ortholog: 15q25Cellular Component: integral to membrane; plasma membrane; synapseMolecular Function: receptor activityBiological Process: nervous system development; cell differentiation
