Anti-SEMA4B Antibody (PACO56170)

Antibody Name:	SEMA4B Antibody (PACO56170)
Antibody SKU:	PACO56170
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:100-1:300, IF:1:50-1:200
Species Reactivity:	Human, Rat
Immunogen:	Recombinant Human Semaphorin-4B protein (163-253AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: Rat luing tissue. All lanes: SEMA4B antibody at 6.3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 93, 82 kDa. Observed band size: 93 kDa.
	IHC image of PACO56170 diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
	IHC image of PACO56170 diluted at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:	Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
Synonyms:	Semaphorin-4B, SEMA4B, KIAA1745 SEMAC

UniProt Protein Function:	SEMA4B iso3: a single-pass type I membrane protein that inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Two alternatively spliced human isoforms have been described.Protein type: Cell surface; Membrane protein, integralChromosomal Location of Human Ortholog: 15q25Cellular Component: integral to membrane; plasma membrane; synapseMolecular Function: receptor activityBiological Process: nervous system development; cell differentiation
UniProt Protein Details:
NCBI Summary:
UniProt Code:	Q9NPR2
NCBI GenInfo Identifier:	29840873
NCBI Gene ID:	10509
NCBI Accession:	Q9NPR2.3
UniProt Secondary Accession:	Q9NPR2,Q6UXE3, Q8WVP9, Q96FK5, Q9C0B8, Q9H691, Q9NPM8 Q9NPN0
UniProt Related Accession:	Q9NPR2
Molecular Weight:	81,403 Da
NCBI Full Name:	Semaphorin-4B
NCBI Synonym Full Names:	sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B
NCBI Official Symbol:	SEMA4B
NCBI Official Synonym Symbols:	SemC; SEMAC
NCBI Protein Information:	semaphorin-4B; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4B
UniProt Protein Name:	Semaphorin-4B
UniProt Synonym Protein Names:
Protein Family:	Semaphorin
UniProt Gene Name:	SEMA4B
UniProt Entry Name:	SEM4B_HUMAN

Antibodies

Secondary Antibody

Biological Role of GLP-1