The SEMA4A Polyclonal Antibody (CAB17205) is a valuable tool for research involving SEMA4A, a cell surface protein with diverse functions in cell signaling, development, and immune responses. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By targeting the SEMA4A protein, this antibody enables the detection and analysis of SEMA4A expression in a variety of cell types, making it an essential tool for studies in immunology, neurobiology, and cancer research.
SEMA4A, also known as semaphorin-4A, plays a crucial role in mediating cell-cell interactions and signaling pathways involved in immune responses and neuronal development. Its involvement in regulating immune cell migration, angiogenesis, and axon guidance makes it a promising target for investigating diseases such as cancer, autoimmune disorders, and neurological conditions. Understanding the functions of SEMA4A is essential for uncovering its potential as a therapeutic target or biomarker in various disease contexts.
Product Name:
SEMA4A Rabbit Polyclonal Antibody
SKU:
CAB17205
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 320-580 of human SEMA4A (NP_071762.2).
This gene encodes a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is an activator of T-cell-mediated immunity and suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
64218
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HT-29 cells, using SEMA4A Rabbit pAb (CAB17205) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
