The SELPLG Polyclonal Antibody (CAB1660) is a valuable tool for researchers studying the SELPLG protein, also known as P-selectin glycoprotein ligand 1. This cell surface glycoprotein plays a crucial role in immune response and inflammation, particularly in the adhesion of leukocytes to endothelial cells.Raised in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications. It binds specifically to the SELPLG protein, allowing for accurate detection and analysis in a variety of cell types. This makes it an ideal choice for studies focusing on immunology, inflammation, and vascular biology.
The SELPLG protein is known to be involved in immune cell trafficking and may play a role in diseases such as atherosclerosis, inflammatory bowel disease, and autoimmune disorders. By understanding the function of SELPLG, researchers can uncover potential therapeutic targets for treating these conditions.Overall, the SELPLG Polyclonal Antibody (CAB1660) is a valuable tool for uncovering the role of SELPLG in immune responses and inflammatory processes, making it a crucial component in research efforts aimed at developing new treatments for a range of diseases.
Product Name:
SELPLG Rabbit Polyclonal Antibody
SKU:
CAB1660
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 348-412 of human CD162/PSGL-1 (NP_002997.2).
Sequence:
KGHM YPVR NYSP TEMV CISS LLPD GGEG PSAT ANGG LSKA KSPG LTPE PRED REGD DLTL HSFL P
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
CLA; CD162; PSGL1; PSGL-1; CD162/PSGL-1
Positive Sample:
Jurkat
Conjugate:
Unconjugated
Cellular Localization:
Membrane, Single-pass type I membrane protein.
Calculated MW:
43kDa
Observed MW:
120kDa
This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
6404
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CD162/PSGL-1 antibody (CAB1660) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
