The SEC24D Monoclonal Antibody (ABM50009) is a vital tool for researchers studying SEC24D, a protein involved in the COPII vesicle trafficking pathway. This antibody, produced using hybridoma technology, is highly specific for SEC24D and has been validated for use in various applications, including Western blot and immunofluorescence.SEC24D is a key component of the COPII complex, responsible for transporting proteins from the endoplasmic reticulum to the Golgi apparatus. Dysfunction in this pathway can lead to various diseases, making SEC24D a crucial target for investigation in the fields of cell biology and molecular biology.
By using the SEC24D Monoclonal Antibody, researchers can accurately detect and analyze SEC24D expression in different cell types and tissues, providing valuable insights into its role in protein trafficking and potential implications in disease. This antibody is a valuable resource for advancing our understanding of cellular processes and developing targeted therapies for related disorders.
Product Name:
SEC24D Monoclonal Antibody
SKU:
CAB21139
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human SEC24D (NP_001304995.1).
Cytoplasm, Endoplasmic reticulum membrane, Golgi apparatus membrane, perinuclear region
Calculated MW:
113kDa
Observed MW:
115kDa
The protein encoded by this gene is a member of the SEC24 subfamily of the SEC23/SEC24 family, which is involved in vesicle trafficking. The encoded protein has similarity to yeast Sec24p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. This gene product is implicated in the shaping of the vesicle, and also in cargo selection and concentration. Mutations in this gene have been associated with Cole-Carpenter syndrome, a disorder affecting bone formation, resulting in craniofacial malformations and bones that break easily. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
9871
Clone Number:
ARC3016
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using SEC24D Rabbit mAb (CAB21139) at1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
