The SDHB Polyclonal Antibody is a vital tool for researchers studying SDHB, a crucial protein involved in cellular respiration and energy production. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the SDHB protein, researchers can accurately detect and analyze SDHB expression in a variety of cell types.SDHB is a key subunit of the succinate dehydrogenase (SDH) enzyme complex, playing a crucial role in the electron transport chain and oxidative phosphorylation.
Mutations in the SDHB gene have been linked to various cancers and mitochondrial disorders, highlighting the importance of studying this protein in research settings. The SDHB Polyclonal Antibody provides researchers with a reliable tool for investigating the role of SDHB in health and disease, making it an essential component in studies related to cancer biology, metabolism, and mitochondrial function.
Product Name:
SDHB Polyclonal Antibody
SKU:
CAB22020
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 29-280 of human SDHB (NP_002991.2).
This tumor suppressor gene encodes the iron-sulfur protein subunit of the succinate dehydrogenase (SDH) enzyme complex which plays a critical role in mitochondria. The SDH enzyme complex is composed of four nuclear-encoded subunits. This enzyme complex converts succinate to fumarate which releases electrons as part of the citric acid cycle, and the enzyme complex additionally provides an attachment site for released electrons to be transferred to the oxidative phosphorylation pathway. The SDH enzyme complex plays a role in oxygen-related gene regulation through its conversion of succinate, which is an oxygen sensor that stabilizes the hypoxia-inducible factor 1 (HIF1) transcription factor. Sporadic and familial mutations in this gene result in paragangliomas, pheochromocytoma, and gastrointestinal stromal tumors, supporting a link between mitochondrial dysfunction and tumorigenesis. Mutations in this gene are also implicated in nuclear type 4 mitochondrial complex II deficiency.
Purification Method:
Affinity purification
Gene ID:
6390
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using SDHB Rabbit pAb (CAB22020) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 0.5s.
