The SDHB Polyclonal Antibody (CAB10821) is a specialized research tool designed for investigating SDHB, a crucial protein involved in mitochondrial function and energy production. This antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it a reliable tool for Western blot applications.SDHB plays a key role in the electron transport chain within the mitochondria, essential for generating ATP and maintaining cellular energy balance. Dysregulation of SDHB has been linked to various diseases, including mitochondrial disorders, neurodegenerative diseases, and cancer.
By targeting SDHB with this antibody, researchers can probe its expression and activity in different cell types, shedding light on its functions in physiological and pathological processes.Given the importance of SDHB in cellular metabolism and disease development, this polyclonal antibody is valuable for studies in biochemistry, cell biology, and cancer research. Its ability to detect and analyze SDHB protein levels in various experimental settings makes it a versatile tool for unraveling the intricate mechanisms underlying mitochondrial dysfunction and its implications for health and disease.
Product Name:
SDHB Rabbit Polyclonal Antibody
SKU:
CAB10821
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 29-280 of human SDHB (NP_002991.2).
This tumor suppressor gene encodes the iron-sulfur protein subunit of the succinate dehydrogenase (SDH) enzyme complex which plays a critical role in mitochondria. The SDH enzyme complex is composed of four nuclear-encoded subunits. This enzyme complex converts succinate to fumarate which releases electrons as part of the citric acid cycle, and the enzyme complex additionally provides an attachment site for released electrons to be transferred to the oxidative phosphorylation pathway. The SDH enzyme complex plays a role in oxygen-related gene regulation through its conversion of succinate, which is an oxygen sensor that stabilizes the hypoxia-inducible factor 1 (HIF1) transcription factor. Sporadic and familial mutations in this gene result in paragangliomas, pheochromocytoma, and gastrointestinal stromal tumors, supporting a link between mitochondrial dysfunction and tumorigenesis. Mutations in this gene are also implicated in nuclear type 4 mitochondrial complex II deficiency.
Purification Method:
Affinity purification
Gene ID:
6390
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SDHB Rabbit pAb (CAB10821) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
