The SCUBE2 Antibody (PAC057448) is a reliable tool for researchers studying SCUBE2, a protein involved in various cellular processes and signaling pathways. This polyclonal antibody is raised in rabbits and has been validated for use in multiple applications, including Western blot and immunofluorescence. By specifically binding to SCUBE2, this antibody allows for the accurate detection and analysis of SCUBE2 levels in different cell types.SCUBE2 is known for its role in cell adhesion, cell migration, and signal transduction, making it a key player in developmental biology and cancer research.
Its overexpression has been linked to tumor progression and metastasis, highlighting the importance of studying SCUBE2 in understanding disease mechanisms. By using the SCUBE2 Antibody, researchers can gain valuable insights into the functions of SCUBE2 and its potential implications in various pathologies.
IHC image of PACO57448 diluted at 1:400 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot. Positive WB detected in: HT29 whole cell lysate, Rat spleen tissue. All lanes: SCUBE2 antibody at 8.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 110, 107, 89 kDa. Observed band size: 110, 89 kDa.
Background:
cell surface, extracellular region, extracellular space, cell differentiation, signal transduction
Synonyms:
Signal peptide, CUB and EGF-like domain-containing protein 2 (Protein CEGP1) (Scube/You), SCUBE2, CEGP1
UniProt Protein Function:
SCUBE2: 3 isoforms of the human protein are produced by alternative splicing.Protein type: Secreted; Secreted, signal peptideChromosomal Location of Human Ortholog: 11p15.3Cellular Component: extracellular regionMolecular Function: calcium ion binding
